Stem Cells
Permanent URI for this collectionhttps://hdl.handle.net/1807/2326
null
Browse
Recent Submissions
Item The cellular basis of the genetically determined hemopoietic defect in anemic mice of genotype Sl/Sld(American Society of Hematology, 1965-10-01) McCulloch, Ernest A. ; Siminovitch, Louis ; Till, James E. ; Russell, Elizabeth S. ; Bernstein, Seldon E.Summary: The proliferation and function of hemopoietic cells derived from genetically anemic Sl/Sld mice have been studied by the use of cell transplantation technics. It was found that marrow cells derived from anemic Sl/Sld or Sld/Sld mice, when implanted into heavily irradiated mice of genotype +sl/+sl, are capable of forming macroscopic spleen colonies, with approximately the same frequency as cells derived from normal +sl/+sl mice. Marrow cells derived from Sl/Sld animals were tested for their capacity to cure the anemia of W/Wv mice and were found to implant as rapidly and to have as long-lasting a beneficial effect as did marrow cells from +sl/+sl mice. The radiosensitivity of the colony-forming ability of marrow cells from Sl/Sld mice was found to be similar to that found previously for +sl/+sl marrow cells, although the anemic animals are known to be more sensitive to total-body irradiation than are their normal littermates. Marrow cells from +sl/+sl mice were found to proliferate more slowly in irradiated mice of genotype Sl/Sld than in irradiated +sl/+sl littermates, even when the anemic and the normal mice were joined in parabiosis. These observations indicate that the hemopoietic colony-forming cells in mice of genotype Sl/Sld are normal, but fail to function adequately because the tissues of mice of this genotype are unable to provide sufficient support for proliferation and differentiation of these progenitor cells.Item The radiation sensitivity of normal mouse bone marrow cells, determined by quantitative marrow transplantation into irradiated mice(Radiation Research Society, 1960-07) McCulloch, Ernest A. ; Till, James E.SUMMARY: 1. A technique for measuring the number of viable cells in a suspension of bone marrow by quantitative transplantation into supralethally irradiated mice has been described. 2. The technique was used to measure the radiation sensitivity of normal mouse bone marrow cells and yielded a result of 105 ± 24 rads as the D37 for marrow cells.Item A direct measurement of the radiation sensitivity of normal mouse bone marrow cells(Radiation Research Society, 1961-02) Till, James E. ; McCulloch, Ernest A.SUMMARY: 1. A direct technique for the measurement of the number of cells in a bone marrow suspension capable of continued proliferation is described. 2. The technique was used to measure the radiation sensitivity of normal mouse bone marrow cells and yielded a Do of 115 ± 8 rads.Item Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells(Nature Publishing Group, 1963-02-02) Becker, Andrew J. ; McCulloch, Ernest A. ; Till, James E.Item The distribution of colony-forming cells among spleen colonies(Wiley-Liss, Inc., A Wiley Company, 1963-12) Siminovitch, Louis ; McCulloch, Ernest A. ; Till, James E.Item A stochastic model of stem cell proliferation, based on the growth of spleen colony-forming cells(National Academy of Sciences (USA), http://www.pnas.org/, 1964-01) Till, James E. ; McCulloch, Ernest A. ; Siminovitch, LouisItem Cytological evidence for a relationship between normal hematopoietic colony-forming cells and cells of the lymphoid system(The Rockefeller University Press, 1968-03-01) Wu, Alan M. ; Till, James E. ; Siminovitch, Louis ; McCulloch, Ernest A.Item Physical separation of hemopoietic stem cells differing in their capacity for self-renewal(The Rockefeller University Press, 1969-07) Worton, Ronald G. ; McCulloch, Ernest A. ; Till, James E.Item Spleen-colony formation in anemic mice of genotype WWv(American Association for the Advancement of Science, 1964-05-15) McCulloch, Ernest A. ; Siminovitch, Louis ; Till, James E.The hemopoietic cells from anemic mice of genotype WWv are less able by 200-fold to take part in colony formation in the spleen than cells from the normal littermates of genotype ww. The genetic defect shows itself in the colony-forming cells, since cells from normal littermate mice form colonies in the spleens of unirradiated mice of genotype WWv. Use of animals of genotype WWv as recipients improves the spleen-colony method by removing bias resulting from the death of irradiated recipients.