Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells
Date
1963-02-02
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Nature Publishing Group
Abstract
Description
Normal mouse blood-forming tissue contains a class of cells which, on being transplanted into heavily irradiated mice, can proliferate and form macroscopic colonies in the spleen. Often, within a given colony, the cells which are observed indicate that differentiation is occurring along three lines, into cells of the erythrocytic, granulocytic and megakaryocytic series, respectively. Unique radiation-induced marker chromosomes were used to obtain direct cytological evidence that the spleen colonies are clones. Thus, the advantages inherent in the clonal approach to studies of the genetic and physiological properties of cells are available for investigations on hæ¡topoiesis. Reproduced from Nature 1963(2 Feb); 197(4866): 452-454. The Nature website is www.nature.com
Keywords
bone marrow cells, CFU, chromosome markers, colony-forming units, cytology, hematopoietic stem cell transplantation, hematopoietic stem cells, hematopoietic progenitor cells, inbred mouse strains, mouse marrow, spleen colonies
Citation
Nature 1963(2 Feb); 197(4866): 452-454.
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