Physical separation of hemopoietic stem cells differing in their capacity for self-renewal
Date
1969-07
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Publisher
The Rockefeller University Press
Abstract
Description
The stem cells in mouse hemopoietic tissues that may be detected by their ability to form macroscopic splenic colonies possess the capacity for self-renewal and the capacity for differentiation. They express both these properties to widely varying degrees. The possibility that colonies derived from individual stem cells differ in composition because of intrinsic differences in the stem cells themselves was tested directly. A physical technique of cell separation, velocity sedimentation, was utilized to fractionate populations of hemopoietic stem cells into subpopulations possessing different sedimentation rates. Tests were made of the capacity for self-renewal of the stem cells present in the different fractions. Stem cells from different fractions were found to renew themselves to different degrees, providing strong evidence that stochastic processes and environmental factors are not the sole sources of heterogeneity in the composition of splenic colonies. Reproduced from The Journal of Experimental Medicine, 1969(Jul); 130(1): 91-103 by copyright permission of The Rockefeller University Press (www.rockefeller.edu/rupress/).
Keywords
bone marrow cells, cell separation, CFU, colony-forming units, hematopoietic stem cells, hematopoietic progenitor cells, mouse marrow, spleen colonies
Citation
J Exp Med 1969(Jul); 130(1): 91-103
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