Department of Laboratory Medicine and Pathobiology

Permanent URI for this collectionhttps://hdl.handle.net/1807/16914

Being the largest department of its kind within Canada as well as among the largest worldwide, the Department of Laboratory Medicine and Pathobiology (LMP) is housed in the many affiliated teaching hospitals as well as within the University of Toronto campus (Banting Institute, Medical Science Buildings, and Forensic Sciences Building). With over 200 primary appointed physicians and scientists as well as over 150 students proceeding towards MSc and PhD, the department contains three divisions; Clinical Biochemistry, Medical Microbiology, and Pathology. Herein, both basic translational and clinically applied research are conducted within well equipped biomedical laboratory facilities.

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Department of Laboratory Medicine and Pathobiology

Affiliated Teaching Hospitals
University Health Network
Sunnybrook Health Sciences Center
Women's College Hospital
Mount Sinai Hospital
St. Micheal's Hospital
The Hospital for Sick Children

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Now showing 1 - 12 of 12

Postprandial Dyslipidemia, Hyperinsulinemia, and Impaired Gut Peptides/Bile Acids in Adolescents with Obesity - Supplemental Files
(2019-12-11) Higgins, Victoria; Agari, Shervin; Hamilton, Jill K.; Wolska, Anna; Remaley, Alan T.; Hartmann, Bolette; Holst, Jens J.; Adeli, Khosrow
Background: Postprandial dyslipidemia strongly associates with obesity and insulin resistance and is an important cardiovascular disease risk factor. Glucagon-like peptides (i.e. GLP-1 and GLP-2) and bile acids have been shown to regulate dietary fat absorption and postprandial lipids in animal models and some human studies. Using healthy adolescents and adolescents with obesity as an experimental model, we aimed to determine the link between postprandial response of GLPs and bile acids to dietary fat and development of postprandial dyslipidemia. Methods: Normal weight (NW) adolescents and those with obesity and mild (OB-MIR) or overt insulin resistance (OB-IR) underwent a 6-hour oral fat tolerance test. The postprandial lipoprotein phenotype profile was determined using various assays, including nuclear magnetic resonance spectroscopy, to characterize lipoprotein particle number, size, lipid content, and apolipoproteins. GLP-1 and GLP-2 were quantified by electrochemiluminescent immunoassays. Total bile acids were measured by an automated enzymatic cycling colorimetric method and the bile acid profile by mass spectrometry. Results: Adolescents with obesity exhibited fasting and postprandial dyslipidemia, particularly augmented postprandial excursion of large triglyceride-rich lipoproteins that worsened with increasing insulin resistance severity. Postprandial GLPs were reduced and inversely correlated with postprandial dyslipidemia. Postprandial bile acids were also diminished, particularly lithocholic acid, a potent stimulator of GLP-1 secretion. Conclusion: Blunted postprandial GLPs and bile acids may contribute to the pathogenesis of disordered lipid and lipoprotein homeostasis. Further investigation is needed to assess their potential utility as early biomarkers for postprandial dyslipidemia in adolescents with obesity and insulin resistance.
Fatal severe acute respiratory syndrome is associated with multiorgan involvement by coronavirus
(University of Chicago Press, 2005) Farcas, G. A. ; Poutanen, S. M. ; Mazzulli, T. ; Willey, B. M. ; Butany, J. ; Asa, S. L. ; Faure, P. ; Akhavan, P. ; Low, D. E. ; Kain, K. C.
Human torovirus: A new nosocomial gastrointestinal pathogen
(University of Chicago Press, 1998) Jamieson, F. B. ; Wang, E. E. L. ; Bain, C. ; Good, J. ; Duckmanton, L. ; Petric, M.
Invasive infections due to a fish pathogen, Streptococcus iniae
(Massachusetts Medical Society, 1997) Weinstein, M. R. ; Litt, M. ; Kertesz, D. A. ; Wyper, P. ; Rose, D. ; Coulter, M. ; McGeer, A. ; Facklam, R. ; Ostach, C. ; Willey, B. M. ; Borczyk, A. ; Low, D. E.
Pseudo-SARS [4] (multiple letters)
(Massachusetts Medical Society, 2003) Johnson, S. ; Patel, M. ; Mullane, K. ; Tsang, K. W. ; Ho, P. L. ; Low, D. E.
Is surveillance for multidrug-resistant enterobacteriaceae an effective infection control strategy in the absence of an outbreak?
(University of Chicago Press, 2002) Gardam, M. A. ; Burrows, L. L. ; Kus, J. V. ; Kus, J. V. ; Low, D. E. ; Conly, J. M. ; Humar, A.
Invasive group A streptococcal infections in Ontario, Canada
(Massachusetts Medical Society, 1996) Davies, H. D. ; Mcgeer, A. ; Schwartz, B. ; Green, K. ; Cann, D. ; Simor, A. E. ; Low, D. E.
Identification of severe acute respiratory syndrome in Canada
(Massachusetts Medical Society, 2003) Poutanen, S. M. ; Low, D.E. ; Henry, B. ; Finkelstein, S. ; Rose, D. ; Green, K. ; Tellier, R. ; Draker, R. ; Adachi, D. ; Ayers, M. ; Chan, A. K. ; Skowronski, D. M. ; Salit, I. ; Simor, A. E. ; Slutsky, A. S. ; Doyle, P. W. ; Krajden, M. ; Petric, M. ; Brunham, R. C. ; McGeer, A. J.
Decreased susceptibility of Streptococcus pneumoniae to fluoroquinolones in Canada
(Massachusetts Medical Society, 1999) Chen, D. K. ; McGeer, A. ; Azavedo, J. C. De ; Low, D. E.
Detection of airborne severe acute respiratory syndrome (SARS) coronavirus and environmental contamination in SARS outbreak units
(University of Chicago Press, 2005) Booth, T.F.; Kournikakis, B.; Bastien, N.; Ho, J.; Kobasa, D.; Stadnyk, L.; Li, Y.; Spence, M.; Paton, S.; Henry, B.; Mederski, B.; White, D.; Low, D. E.; McGeer, A.; Simor, A.; Vearncombe, M.; Downey, J.; Jamieson, F. B.; Tang, P.; Plummer, F.
Viable group A streptococci in macrophages during acute soft tissue infection
(Public Library of Science, 2006) Thulin, P. ; Johansson, L. ; Low, D. E. ; Gan, B. S ; Kotb, M. ; McGeer, A. ; Norrby-Teglund, A.
Outpatient gatifloxacin therapy and dysglycemia in older adults
(Massachusetts Medical Society, 2006) Park-Wyllie, L. Y. ; Juurlink, D. N. ; Kopp, A. ; Shah, B. R. ; Stukel, T. A. ; Stumpo, C. ; Dresser, L. ; Low, D. E. ; Mamdani, M. M.

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