Bioline International Legacy Collection
Permanent URI for this collectionhttps://hdl.handle.net/1807/2
CITD (1993-2002) was a centre designed to facilitate faculty and students with creation of innovative learning and research opportunities through New Media and Information Technology. This space serves as the archive for some of the projects created at the centre.
It contains records from Bioline International 1990-2006.
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Item Open Access A New Species of Kritskyia (Dactylogyridae, Ancyrocephalinae) parasite of urinary bladder of Prochilodus lineatus (Prochilodontidae, Characiformes) from the floodplain of the high Paraná River, Brazil(Fundação Oswaldo Cruz, Fiocruz, 2002-12-31) Ricardo Massato Takemoto; Maria de los Angeles Perez Lizama; Gilberto Cezar PavanelliA new species of Kritskyia inhabits the urinary bladder of the "curimba", Prochilodus lineatus in the floodplain of the high Paraná river. The new species resembles others members of Kritskyia in the following features: haptor lacking anchors and with 14 hooks marginal, posterior male copulatory organ non articulated with the accessory piece and vagina a sclerotized tube. However, it differs from the known species mainly by the shape of the copulatory complex. This is the third endoparasitic monogenean species reported from freshwater Neotropical fish.Item Open Access Age structure of adult mosquito (Diptera: Culicidae) populations from Buenos Aires Province, Argentina(Fundação Oswaldo Cruz, Fiocruz, 1997-12-31) Arnaldo MaciaIn order to detect seasonal trends in the age structure of adult mosquitoes from Buenos Aires province, Argentina, female populations were sampled with CDC traps during 1989-1991 in Punta Lara and La Plata. The mosquitoes were dissected and age-grouped according to ovarian tracheation and ovariolar stages. All Runchomyia paranensis females were parous, suggesting that this species could be autogenous. Aedes albifasciatus showed parous peaks following population peaks, with shorter delays in spring-summmer and longer in fall-winter. Ae. crinifer and Culex dolosus showed wide fluctuations in age structure due to adult emergences during all months. Psorophora ferox showed high population replacement rates. Mansonia indubitans and Ma. titillans have few generations per year during their activity period. This is the first report on age-grading of adults of field mosquito populations from Argentina.Item Open Access Electronic publishing of scientific journals - the Bioline model(AusBiotech, 1997-12-31)Australasian Biotechnology, Volume 7 Number 4, July/August 1997, pp.241-244 Electronic publishing of scientific journals - the Bioline model Barbara Kirsop & Brian Kirsop, Code Number: AU97033 Size of Files: Text: 14.7K Graphics: No associated graphics files Introduction The distribution of scientific information using the Internet is a well established mechanism for most of the major public domain databases (GenBank, EMBL, Swissprot etc). By contrast, the electronic distribution of peer reviewed scientific papers is relatively new. However, the rising cost of scientific publications and the diminishing resources of many libraries has created a situation which is restricting the distribution of the results of scientific and technological research. Copyright 1997 Australian Biotechnology Association Ltd.Item Open Access Effect of incorporating Cassia siamea prunings on maize yield in an alley cropping trial in semiarid Kenya(African Crop Science Society, 1997-12-31)African Crop Science Journal Vol.5. No.2, pp. 201-207, 1997 SHORT COMMUNICATION Effect of incorporating Cassia siamea prunings on maize yield in an alley cropping trial in semiarid Kenya D.N. MUGENDI, B.O. MOCHOGE, C.L. COULSON, C.J. STIGTER and F.K. SANG Code Number: CS97026 Sizes of Files: Text: 22.6K Graphics: Line drawings (gif) - 10.8K ABSTRACT The influence of soil-incorporated Cassia siamea prunings on nutrient status of maize leaves, grain and yield was studied in an alley cropping experiment in semiarid Machakos, Kenya, during the 1988 long and short rainy seasons. The trial was established in 1983 and in all the seasons Katumani composite B maize was sown, except in the short rains of 1988 when Hybrid 511 was planted. Plots consisted of 3 cropped alleys between cassia hedges spaced at 3.6 m apart. The hedges were lopped at the beginning of every season (on-set of rains) and the prunings incorporated into the alleys one day before maize seeds were sown. The control plots had no hedges and hence no prunings were incorporated. Incorporation of prunings into the soil increased nutrient concentration in the maize leaves, grains and soil in the treatment plots compared to the controls. Maize grain yield on a per row basis was also higher in the plots that received prunings application compared to the controls. However, on an area basis, the yield increase was insufficient to compensate for the area lost to the Cassia siamea shrubs. Key Words: Agroforestry, hedge row intercropping, cassia shrubs RESUME L'impact des emondes de Cassia siamea inorpores dans le sol sur la production et la composition minerale des feuilles de mais et des grains a ete etudie dans une experimentation de culture en couloirs. Celle-ci a ete organisee en region semi-aride de Machakos au Kenya pendant les grandes et petites saisons pluvieuses de 1988. L'essai a ete mis en place en 1983. Chaque saison, le compose B du mais connu sous le nom de Katumani etait serre, sauf pendant les petites saisons pluvieuses de 1988 lorsque l'hybride 511 etait plante. Les parcelles etaient constituees de 3 allees cultivees entre les haies de cassia distantes entre elles de 3.6 m. Les haies etaient elaguees au debut de chaque saison pluvieuse. Les emonoles etaient ensuite incorporees dans les allees un jour avant la semence des graines. Les parcelles de contrle n'avaient pas de haies; les emondes n'y etaient donc pas incorporees. L'incorporation des emondes dans le sol des parcelles de traitement augmentait la teneur des elements mineraux nutritifs dans les feuilles et les grains de mais. Cela n'etait donc pas le cas dans les parcelles de contrle. La production des grains de mais par rangee s'est egalement averee plus importante dans les parcelles ou les emondes ont ete appliquees que dans celles de contrle. Cependant, en terme de surface la croissance de la production etait insuffisante pour pouvoir compenser la perte de la surface due a la presence des arbustes de Cassia siamea. Mots Cles: Agroforesterie, culture en cauloir, arbustes de cassia Copyright 1997 The African Crop Science SocietyItem Open Access Changes in Nuclear Phenotype Frequencies Following Sequential Cold Shocks in Triatoma infestans (Hemiptera, Reduviidae)(Fundação Oswaldo Cruz, Fiocruz, 2002-12-31) Silvana G. P. Campos; Vera Lúcia Cortiço Corrêa Rodrigues; Maria Luiza S. MelloThe nuclear phenotypes of Malpighian tubule cells in fifth instar nymphs of Triatoma infestans , one of the most important vectors of Chagas disease, were studied following sequential shocks at 0ºC, separated by intervals of 8 h and 24 h at 30ºC, under conditions of moderate fasting and full nourishment. The insects pertained to colonies reared in the laboratory and originated from domestic specimens collected in the Brazilian states of São Paulo (north) and Minas Gerais (south). Since nuclear phenotypes in this species are affected by single cold shocks, it was expected that these phenotypes could also be changed by sequential shocks. Nuclear phenotypes indicative of mechanisms of cell survival (nuclear fusion and heterochromatin decondensation) and cell death (apoptosis and necrosis) were observed concomitantly in all the conditions tested. Nuclear fusion and heterochromatin decondensation were not found relevant for the presumed acquisition of the cold-hardening response in T. infestans . The decreased frequency of apoptosis and necrosis following sequential cold shocks including under fasting conditions, indicated that tolerance to sequential cold shocks occurred in T. infestans of the mentioned origin.Item Open Access The Role and Effectiveness of Adjunctive Hyperbaric Oxygen Therapy in the Management of Musculoskeletal Disorders(Medknow Publications and Staff Society of Seth GS Medical College and KEM Hospital, Mumbai, India, 2002-12-31) J. Wang; F. Li; J. H. Calhoun; J. T. MaderThe management of musculoskeletal disorders is an increasing challenge to clinicians. Successful treatment relies on a wide range of multidisciplinary interventions. Adjunctive hyperbaric oxygen (HBO) therapy has been used as an orthopaedic treatment for several decades. Positive outcomes have been reported by many authors for orthopaedic infections, wound healing, delayed union and non-union of fractures, acute traumatic ischaemia of the extremities, compromised grafts, and burn injuries. Severe side effects have also been reported with this therapy. To aid in the use of HBO therapy in orthopaedics, we reviewed 43 papers published in the past four decades and summarised the mechanisms, effectiveness, indications and contraindications, side effects, and cost impact of adjunctive hyperbaric oxygen therapy in the management of difficult musculoskeletal disorders. Adjunctive HBO therapy is an effective treatment modality for the management of some severe and refractory musculoskeletal problems. If appropriate candidates are carefully identified, hyperbaric oxygen is a limb- and sometimes life-saving therapy. HBO therapy significantly reduces the length of the patient's hospital stay, amputation rate, and wound care expenses. Thus, it is a cost-effective modality. A clinician must understand the side effects and risks of HBO treatment. Close monitoring throughout the treatment is warranted to minimise the risk to the patients. (J Postgrad Med 2002;48:226-231)Item Open Access RESEARCH NOTE: Helminths Parasites of Eupsophus roseus (Anura: Leptodactylidae) from Southern Chile(Fundação Oswaldo Cruz, Fiocruz, 1999-12-31)Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 94(6): 725-726, Nov./Dec. 1999 RESEARCH NOTE Helminths Parasites of Eupsophus roseus (Anura: Leptodactylidae) from Southern Chile Sonia Puga, Patricio Torres Code Number:OC99219 Key words: helminths - Nematoda - Trematoda -Acanthocephala - Anura - Eupsophus roseus Eupsophus roseus Dumeril & Bibrón, 1841 is a small sized (2.6-9.5 cm) endemic frog of the temperate Nothofagus forests of Southern Chile (JR Formas 1979 Univ Kansas Mus Nat Hist Mongr 7: 341-369). In this species the following parasite endohelminths have been reported: Oswaldocruzia neghmei Puga, 1981, Rudolphitrema chilensis Puga, 1986 (S Puga 1994 Bol Chil Parasitol 49: 81-84) and Centrorhynchus sp. (P Torres & S Puga 1996 Mem Inst Oswaldo Cruz 91: 717-719). Copyright 1999 Fundacao Oswaldo Cruz - FiocruzItem Open Access Accuracy of references in Indian Journal of Surgery(Medknow Publications on behalf of Association of Surgeons of India, 2003-12-31) Anup Mohta; Medha MohtaThe references are an important component of any scientific publication. Accuracy of references is essential for providing credibility to the manuscript, authors and journal. This study found extremely high error rate in the references of the Indian Journal of Surgery. Sincere effort is required on the part of authors and editorial board to improve the citations.Item Open Access FROM THE EXECUTIVE DIRECTOR(AusBiotech, 2001-12-31) Tony CoulepisThese are certainly exciting times for the Australian biotechnology industry, and consequently I am delighted to have the opportunity of being involved and assisting the ABA with the next stage of its development as an industry organisation. My association with the ABA Steering Group highlighted to me the need, as well as the overwhelming support from biotechnology stakeholders, for the ABA to move forward, take up the challenge, and emerge as the Australian biotechnology industry organisation...Item Open Access Economic Analysis Of Maize-Bean Production Using A Soil Fertility Replenishment Product (Prep-Pac) In Western Kenya(African Crop Science Society, 1999-12-31)African Crop Science Journal, Vol. 7. No. 4, pp. 585-590, 1999 Economic Analysis Of Maize-Bean Production Using A Soil Fertility Replenishment Product (Prep-Pac) In Western Kenya Code Number: CS99049 ABSTRACT: The Phosphate Rock Evaluation Project (PREP) is testing PREP-PAC, a soil fertility replenishment product specifically designed to ameliorate nutrient-depleted "patches" symptomatic of the worst maize-bean intercrops of smallholders' fields in western Kenya. PREP-PAC contains two kg Minjingu rock phosphate, 0.2 kg urea, legume seed, rhizobial seed inoculant, seed adhesive and lime pellet, is assembled and is sold for Ksh. 42 (US $ 0.76) and is intended for 25 m2 areas. PREP-PAC was tested on 52 farms in four districts of western Kenya during 1998 and compared with adjacent control plots. Farmers selected either a local bush or climbing variety (cv. Flora) of Phaseolus vulgaris as an intercrop with maize (Zea mays). Use of the combined PREP-PAC and climbing bean package increased maize and bean yields by 0.72 and 0.25 t ha-1, respectively (P < 0.001), resulting in a 161% return on investment. Total revenue from low pH soils (<5.2) was Ksh. 25 for the control and Ksh. 47 for PREP-PAC. In moderate soil pH >(5.3), total revenue was Ksh. 31 for control and Ksh. 68 for PREP-PAC (P < 0.05). Opportunity exists to distribute an affordable soil fertility restoration package among smallhold farmers but the profitability from its use is dependent upon soil conditions and accompanying legume intercrops. KEYWORDS: East African Highlands, nutrient-depleted soil, nutrient replenishment, smallholder farming systems, rock phosphate Copyright 1999, African Crop Science SocietyItem Open Access Biotechnology and human health: present and future applications. The WA Australian Biotechnology Association essay competition(AusBiotech, 1996-12-31)Australasian Biotechnology, Volume 6 Number 5, September/October 1996, pp.304-306 Biotechnology and human health: present and future applications. The WA Australian Biotechnology Association essay competition Code Number: AU96018 Size of Files: Text: 13.8K Graphics: No associated graphics files This competition was held recently and was open to all Year 11 and 12 students in Western Australia. The essay topic was Biotechnology and human health: present and future applications. Copyright 1996 Australian Biotechnology Association Ltd.Item Open Access Characteristics of the Biological Cycle of Lutzomyia evandroi Costa Lima & Antunes, 1936 (Diptera: Psychodidae) under Experimental Conditions(Fundação Oswaldo Cruz, Fiocruz, 2001-12-31)Mem Inst Oswaldo Cruz 2001, Volume 96, Number 6, pp. 883-886 Characteristics of the Biological Cycle of Lutzomyia evandroi Costa Lima & Antunes, 1936 (Diptera: Psychodidae) under Experimental Conditions Maria de Fátima Freire de Melo Ximenes, Janaína Cunha Maciel, Selma Maria Bezerra Jerônimo Code Number: oc01143 ABSTRACT: Lutzomyia evandroi Costa Lima and Antunes, 1936 is found in Rio Grande do Norte, northeastern Brazil, in areas of visceral and mucocutaneous leishmaniasis and follows the same geographic distribution of L. longipalpis. The biological cycle, oviposition, morphological and behavioral characteristics of the species were studied under experimental conditions. The average number of eggs per wild caught female varied from 21 to 50 eggs along the year, with a peak occurring between January and March and another in August, with oviposition lasting for 4 to 12 days. The mean larval phase was 24 days. Ovipositing rates were influenced by rainfall and temperature indexes, with an increase of eggs per oviposition at the beginning and at the end of the rainy season, and a decrease at the peak of the rainy season. KEYWORDS: Lutzomyia evandroi, oviposition, biological cycle Copyright 2001 Mem Inst Oswaldo CruzItem Open Access Evolução dos Conhecimentos sobre Vetores da Doença de Chagas 90 Anos após sua Descoberta(Fundação Oswaldo Cruz, Fiocruz, 1999-12-31) Herman LentItem Open Access Estudio comparativo de dos sistemas de captura de la protena de 24 kD del virus de inmunodeficiencia humana tipo 1(Elfos Scientiae, 2000-12-31)Biotecnología Aplicada 2000, Volume 17, Number 2, pp. 102-104 Estudio comparativo de dos sistemas de captura de la protena de 24 kD del virus de inmunodeficiencia humana tipo 1 Code: ba00024 RESUMEN: Se realiz un estudio comparativo entre los sistemas HIV-1 P24 Antigen ELISA Test (Coulter, EUA) y DAVIH-Ag P24 (Laboratorios DAVIH, Cuba) para la deteccin del antgeno P24 del VIH-1. Mediante ambos sistemas se estudiaron en paralelo 1 039 muestras de suero de donantes de sangre y 323 de individuos seropositivos en diferentes estadios de infeccin por VIH-1. La presencia de antgeno se relacion con el grupo clnico y los niveles de anticuerpos contra la P24. Los resultados mostraron que ambos diagnosticadores son altamente especficos (ms de 99%), con una concordancia de 99,7%. Para la poblacin positiva estudiada, el sistema DAVIH-Ag P24 result ms sensible que el sistema de la firma Coulter. Los por cientos de antigenemia fueron mayores en el grupo de los pacientes de SIDA. Los ttulos de anticuerpos contra la P24 resultaron ser bajos o no reactivos en todos los sueros con antigenemia a esta protena. Se concluy que el sistema DAVIH-Ag P24 puede ser utilizado para el seguimiento de personas infectadas con el VIH-1 como marcador pronstico de la infeccin, y para el pesquisaje de la P24 en los bancos de sangre. PALABRAS CLAVES: antigenemia, ELISA, marcadores de progresin, P24, seroconversin, VIH-1 ABSTRACT: Comparative Study of Two Capture Systems for the 24-kD protein of Human Immunodeficiency Virus. A comparative study between HIV-1 Antigen ELISA Test (Coulter, USA) and DAVIH-Ag P24 (DAVIH Laboratories, Cuba) was performed for the detection of HIV-1 P24 antigen. A total of 1039 serum samples of blood donors. sera were studied, in addition to 323 sera from seropositive individuals in different stages of the disease. The presence of antigen was associated with the clinical group and the levels of antibodies against P24. Both systems showed a high specificity (greater than 99%) with a concordance of 99.7%. DAVIH-Ag P24 system showed a sensitivity (58/31) greater than Coulter system when it was tested in the positive population. Percents of antigenemia were greater in the AIDS group. Antibody titers against P24 were low or absent in all the sera with antigenemia to this protein. It was concluded that DAVIH-Ag P24 system can be used in the follow-up of HIV-1 infected individuals as a progression marker of the infection, and to screen P24 in blood banks. KEYWORDS: antigenemia, ELISA, HIV-1, P24, progression markers, seroconversion Copyright 2000 Elfos ScientiaeItem Open Access Schistosomiasis Vaccine Development: Progress and Prospects(Fundação Oswaldo Cruz, Fiocruz, 1998-12-31) N. R. BergquistThe undisputed, worldwide success of chemotherapy notwithstanding, schistosomiasis continues to defy control efforts in as much rapid reinfection demands repeated treatment, sometimes as often as once a year. There is thus a need for a complementary tool with effect for the longer term, notably a vaccine. International efforts in this direction have been ongoing for several decades but, until the recombinant DNA techniques were introduced, antigen production remained an unsurmountable bottleneck. Although animal experiments have been highly productive and are still much needed, they probably do not reflect the human situation adequately and real progress can not be expected until more is known about human immune responses to schistosome infection. It is well-known that irradiated cercariae consistently produce high levels of protection in experimental animals but, for various reasons, this proof of principle cannot be directly exploited. Research has instead been focussed on the identification and testing of specific schistosome antigens. This work has been quite successful and is already at the stage where clinical trials are called for. Preliminary results from coordinated in vitro laboratory and field epidemiological studies regarding the protective potential of several antigens support the initiation of such trials. A series of meetings, organized earlier this year in Cairo, Egypt, reviewed recent progress, selecteded suitable vaccine candidates and made firm recommendations for future action including pledging support for large-scale production according to good manufacturing practice (GMP) and Phase I trials. Scientists at the American Centers for Disease Control and Prevention (CDC) have drawn up a detailed research plan. The major financial support will come from USAID, Cairo, which has established a scientific advisory group of Egyptian scientists and representatives from current and previous international donors such as WHO, NIAID, the European Union and the Edna McConnell Clark Foundation.Item Open Access The state of the art of paleoparasitological research in the Old World(Fundação Oswaldo Cruz, Fiocruz, 2003-12-31) Françoise Bouchet; Stéphanie Harter; Matthieu Le BaillyPaleoparasitology in the Old World has mainly concerned the study of latrine sediments and coprolites collected from mummified bodies or archaeological strata, mostly preserved by natural conditions. Human parasites recovered include cestodes, trematodes, and nematodes. The well preserved conditions of helminth eggs allowed paleoepidemiological approaches taking into account the number of eggs found by archaeological stratum. Tentatively, sanitation conditions were assessed for each archaeological period.Item Open Access Large-scale Production of recombinant Hepatitis B Surface Antigen from Pichia pastoris(Elfos Scientiae, 2000-12-31)Biotecnologia Aplicada 2000; Vol. 17 No. 1, pp. 52 Large-scale Production of recombinant Hepatitis B Surface Antigen from Pichia pastoris Eugenio Hardy, Eduardo Martínez, David Diago, Raú Díaz, Daniel González, Luis Herrera Code Number: BA00016 Introduction An downstream process for the purification of HBsAg produced in Pichia pastoris was established first at laboratory scale [1] and further scaled-up from about 250- to 500-fold [2]. However, the effectiveness of the key steps for the large-scale production of P. pastoris-derived HBsAg had not been described yet. We analyze here the performance of our production technology in terms of its ability to both render a highly pure HBsAg and remove most of the intrinsic (yeast total proteins, nucleic acids, carbohydrates, lipids) and extrinsic (immunopurification released immunoglobulin [lg] G, endotoxin) contaminants. The results obtained verified that this technology satisfies most of the World Health Organization (WHO) requirements for the safe purification of yeast-derived, biologically-active HBsAg particles. Consequently, the vaccine (HEBERBIOVAC HB, Heberbiotec SA, Cuba), which is formulated with P. pastoris-derived HbsAg, has proven to be safe and efficacious, providing protection against hepatitis B infection [3] Copyright 2000 Elfos ScientiaeItem Open Access Nueva Metodología Para La Evaluación De Proyectos De I+D En Biotecnología(Elfos Scientiae, 1999-12-31)Biotecnología Aplicada 1999;Vol. 16, No. 1 : 53-59 Nueva Metodología Para La Evaluación De Proyectos De I+D En Biotecnología Rubén Ramos Code Number: BA99012 ABSTRACT A methodology for research project evaluation was made in the Center for Genetic Engineering and Biotechnology (CIGB, Havana, Cuba), which allowed to establish priorities among a high variety of projects related with different sectors and basic, applied and technologic innovation profiles. This methodology, used in the CIGB, contributed to a better organization of the research process, as well as to a better design of the already conceived projects. Keywords: biotechnology, evaluation, proyects RESUMEN En el Centro de Ingeniería Genética y Biotecnología (CIGB, Habana, Cuba), se elaboró una metodología para la evaluación de proyectos de investigación en biotecnología, la cual permitió establecer prioridades entre una amplia gama de proyectos relacionados con diferentes sectores y de perfiles básico, aplicado y de innovación tecnológica. Esta metodología, utilizada en el CIGB, contribuyó a una mejor organización del proceso de investigación, así como a un mejor diseño de los proyectos de investigación confeccionados. Palabras claves: biotecnología, evaluación, proyectos Copyright 1999 Elfos ScientiaeItem Open Access Molecular characterization of the potato leafroll luteovirus and developmentof genetically engineered resistance(Elfos Scientiae, 1996-12-31)Biotechnologia Aplicada 1996; Vol 13, No.2 L Kawchuk RR Martin and DR Lynch Code Number: BA96047 Sizes of Files: Text: 3.9K Graphics: No associated graphics files Introduction Potato leafroll luteovirus (PLRV) is difficult to control and is responsible for significant worldwide economic losses in potato (Solanum tuberosum L.). In addition to reducing yields, PLRV reduces the quality of several potato varieties by causing net necrosis of the tubers. Luteoviruses are phloem-limited and transmitted by aphids in a circulative nonpropagative manner. PLRV particles are composed of a single-stranded positive-sense RNA molecule and 180 identical coat protein subunits. Copyright 1996 Elfos ScientiaeItem Open Access Megalencephalic leukoencephalopathy with subcortical cysts: MRI and proton spectroscopic features(Medknow Publications on behalf of the Neurological Society of India, 2003-12-31) H. S. Chandrashekar; A. S. Guruprasad; P. N. Jayakumar; S. G. Srikanth; A. B. TalyMegalencephalic leukoencephalopathy with subcortical cysts (MLC) is a rare, recently described disease.1 This disorder, reported predominantly in infants and children, was originally described by van der Knaap as 'leukoencephalopathy with swelling and a discrepantly mild clinical course'. The gene locus for this autosomal recessive disease has been assigned to chromosome 22qtel, and a gene has been identified, presently called MLC1.2,3 It is characterized clinically by a large head and mild neurological symptoms such as ataxia, spasticity and remarkably slow course of functional deterioration. The MRI features described include 'swollen white matter' with large symmetrical cystic changes in the cerebral hemispheres.1,2,4 The MRS findings in this disorder include mild to moderate decreases in the NAA to choline and choline to creatine ratios.5 We report a child with this disease and discuss the MRI and MRS features.